Western Blotting

Introduction The Western blot is a cornerstone technique in molecular biology and biochemistry, allowing researchers to identify and quantify specific proteins within a complex mixture. After probing a Western blot with an antibody, a world of possibilities can unfold if you have the ability to “strip” the blot. Western blot stripping involves removing the bound … Read more

Introduction Western blotting, a cornerstone technique in molecular biology, allows researchers to identify and quantify specific proteins within a complex mixture. The procedure involves separating proteins by size using gel electrophoresis, transferring them to a membrane, and then probing with specific antibodies to detect the protein of interest. However, the ability to reuse a Western … Read more